SQSTM1 and infection: Since OPTN and p62 are known to function cooperatively in xenophagy of Salmonella enterica [22–24], we next asked if double deficiency of Optn and p62 in zebrafish resulted in an increased infection burden compared to single mutation of either optn or p62. No additive effect on the infection burden was observed when p62 MO was injected into optn mutant embryos or optn MO into p62 mutant embryos (Fig 4E).