MAP1LC3A and infection: To examine whether ubiquitin interacts with Mm and Lc3 during infection of zebrafish, we infected embryos at 28 hours post fertilization (hpf) and performed immunostaining for ubiquitin at 1, 2, and 3 days post infection (dpi), time points at which the early stages of tuberculous granuloma formation can be observed (Fig 1A).