TNF and neoplasm: We subsequently performed coculture assays against Raji tumor cells and measured IL-6 and TNF-α production at 48 h and found that CD4-selected CAR-T cells produced 71% and 76% higher levels of IL-6 and TNF-α compared with unselected CAR-T cells, whereas CD8-selected CAR-T cells produced 99% and 91% less of these molecules, respectively (Fig. 7a).