As a first step toward confirming necroptosis in ALS mice, we compared Ripk1, Ripk3, and Mlkl expression levels by qPCR in the lumbar spinal cord of Tg SOD1G93A mice at two time points, ranging from early-symptomatic (i.e., 12 weeks of age) to near end-stage paralysis (i.e., 15 weeks of age; hereafter referred to as symptomatic), and in age-matched Tg SOD1WT mice and NTg littermates. This evidence concerns the gene MLKL and amyotrophic lateral sclerosis.