Although both of mentioned factors (IL-12 and shRNA for TGF-β1) are very important for effective reactivation of immune response to fight cancer, our research revealed that the influence of TEx MC38/shTGFβ1 on the activation of specific antitumor response as well as reduction of immunosuppression in TME is significantly higher than the effect of treatment with TEx MC38/IL12. This evidence concerns the gene TGFB1 and cancer.