In addition, we confirmed the intrinsic role of TCF1 in the regulation of CD8 T cells in the Tcf7fl/fl-ERT2Cre system via a bone marrow chimera model, which was treated with tamoxifen before (Strategy II) or after (Strategy I) Cl13 infection as described above (Supplementary Figure 3B). The gene discussed is CD8A; the disease is infection.