Considering that 4T1 are p53-deficient breast cancer cells (33), a high NO level, promoted by the NF-κB signalling pathway in the tumour of untreated 4T1-challenged mice, may contribute positively to the tumour progression and metastasis by inducing expression of c-myc, VEGF and MMP-9 genes. This evidence concerns the gene MMP9 and neoplasm.