ADAMTS9 and cleft palate: Adamts9Gt/Gt embryos also had cleft palate (Supplementary Fig. 4i–l), and further Adamts9 dosage reduction via combination of Adamts9Gt with the inactivating Adamts9Del allele (Adamts9Gt/Del) resulted in failure to undergo rotation, an open neural tube and death by E10.5 (Supplementary Fig. 4m–p).