To do so, we used the A549 (non-small cell lung cancer (NSCLC)), the ACHN (renal cell carcinoma derived from pleural effusion), and the immortalized breast MCF10A cell lines treated, or not, with transforming growth factor beta (TGFβ)/tumor necrosis factor alpha (TNFα) to induce EMT6. The gene discussed is TNF; the disease is Pleural effusion.