By up-regulating CD44 expression, subsequently increasing the phosphorylation level of GSK3β, decreasing the phosphorylation level of β-catenin, enhancing nuclear accumulation of β-catenin, and increasing the transcriptional activity of β-catenin/TCF, TRIM29 activates the Wnt/β-catenin signaling pathway to promote EMT in CRC metastasis. The gene discussed is TRIM29; the disease is colorectal carcinoma.