Paguirigan and coworkers have used single cell genetics (multiplexed quantitative PCR) to investigate the patterns of segregation of two concurrent mutations in AML, FLT3-ITD and NPM1 mutations, showing that mutations of FLT3 and NPM1 occur in both homozygous and heterozygous states, distributed among at least 9 different clonal populations in all AML samples analyzed, thus indicating more subclonal heterogeneity that could be inferred from analysis of the bulk population [51]. This evidence concerns the gene NPM1 and acute myeloid leukemia.