As shown in Figure 4b,c, cells in high-grade tumors were almost all rapidly proliferating (88 ± 7% of tumor positive for Ki-67) and 19 ± 6% of the tumor was hypoxic (positive for PIM) as compared to low-grade tumors (22 ± 6% positive for Ki-67, 7 ± 5% for PIM) and normal TRAMP prostates (0.3 ± 0.2% positive for Ki-67, 0% positive for PIM). This evidence concerns the gene MKI67 and neoplasm.