In this study, we applied a metabolic imaging approach with a new dual-agent hyperpolarized (HP) 13C magnetic resonance imaging (MRI) exam to investigate the interplay between prostate cancer metabolism and the microenvironment and to elucidate the functional role of LDHA in prostate cancer progression and metastases using the transgenic adenocarcinoma of mouse prostate (TRAMP) model. Here, LDHA is linked to prostate cancer.