On the other hand, Aoto et al., in a study focused on detection of biomarkers of ICD, specifically CRT and HMGB1, in pretreatment biopsy specimens and surgically resected specimens taken from either patients with breast cancer (n = 52) or esophageal squamous cell carcinoma (ESCC, n = 8), who had been treated with neo-adjuvant chemotherapy (NAC), reported less convincing findings [77]. This evidence concerns the gene HMGB1 and breast carcinoma.