The activities of G6PD and TKT, key PPP enzymes, were increased in cancer cells.29 In this work, we found that the two key PPP enzymes, G6PD and TKT, were up‐regulated in Nrf2‐overexpressed MCF‐7 and MDA‐MB‐231 cells, whereas ectopic overexpression of Keap1 and Nrf2 knockdown counteracts the increase of G6PD and TKT expressions in breast cancer cells. This evidence concerns the gene G6PD and breast cancer.