The results suggest that miR-195-5p negatively regulates NOTCH2, Ad-NOTCH2, and Vimentin levels in CRC cells, while E-cadherin was positively regulated by miR-195-5p, suggesting negative regulations of miR-195-5p on the NOTCH2 pathway and EMT in CRC cells. The gene discussed is VIM; the disease is colorectal carcinoma.