EGFR and lung carcinoma: Also, the allele frequencies of EGFR mutations detected with MBS were comparable between the two libraries across a wide range from around 1 to 50%, including 0.6% of EGFR mutation for the second library of #2351 (Fig. 1c), with significant correlation between the two libraries (R2 = 0.989, Fig. 1d), and thus demonstrating the reproducibility of MBS in quantifying allele frequency of EGFR mutations in lung cancer.