We showed that sPD-L1 and sPD-1 levels before and after CRT were not significantly associated with pathological response to CRT, such as ypT, ypN, and TRG, and suggest that neither sPD-L1 nor sPD-1 is useful to predict pathological response to CRT in rectal cancer. This evidence concerns the gene SPDL1 and rectal cancer.