Real time qPCR revealed a threefold increase in ENG mRNA (CF 3.5 ± 1.8 vs. non‐CF: 1.0 ± 0.4, n = 5; P < 0.05; Fig. 2A), a twofold increase in the representative TGF‐β signal PAI‐1 mRNA (CF 2.2 ± 0.3 vs. non‐CF: 1.0 ± 0.2, n = 5; P < 0.01; Fig. 2B) and a 1.5‐fold increase in TGF‐β1 mRNA (CF 1.5 ± 0.3 vs. non‐CF: 1.0 ± 0.3, n = 5; P < 0.05, data not shown) in lung homogenates of CF compared to non‐CF lung indicating that ENG and TGF‐β signaling (PAI‐1) are increased at the transcription level. This evidence concerns the gene ENG and cystic fibrosis.