Two key preclinical transgenic mouse models were used to support the progression of this therapy to clinical development; subretinal injection of RGX-314 in rho/VEGF mice showed a dose-dependent suppression of subretinal neovascularization, and in a more severe model of nAMD and retinal detachment, the Tet/Opsin/VEGF mouse model, there was a significant reduction in retinal detachment (222). Here, VEGFA is linked to retinal detachment.