How many signal proteins in the Hippo pathway readily crosstalk with proteins from Wnt, TGF-β, and ERK pathways? Tumor suppressor WWOX interacts with these pathways. Can WWOX act as a modulator for optimizing the signaling outcomes?Lacking a real-time mode for cellular signaling pathways is hard to unravel the hidden events (e.g., promoter activation, protein/protein interactions, and etc.). For a signaling with so many branches in the downstream, or a signaling with many upstream stimulators, is there a dominant signal flow? This evidence concerns the gene TGFB1 and neoplasm.