MICA and osteosarcoma: These NKG2D CAR engineered primary NK cells through retroviral transduction showed significantly enhanced in vitro cytotoxicity against a variety of solid tumor cell lines that express NKG2D ligands MICA/B such as the osteosarcoma cell lines U-2 OS, MG-36, HOS, the prostate carcinoma cell lines DU 145 and PC-3, and the rhabdomyosarcoma cell line RH36 (196) and significantly reduced tumor burden in osteosarcoma xenografted NSG mice compared to mock NK cells (196).