To determine whether miR-325-3p overexpression protected HCC cells from HBV-induced apoptosis in an AQP5-dependent manner, we cotransfected Huh7–1.3 and HepG2.2.15 cells with miR-325-3p mimic and pcDNA-AQP5. This evidence concerns the gene AQP5 and hepatocellular carcinoma.