PFKFB1 and Insulin resistance: Genes of interest that were downregulated (n = 24) over the course of the entire experiment included those associated with inflammation and insulin resistance (CD44), stress and hormone responses (DRG1), local production of triiodothyronine (DIO2), regulation of glycolysis (PFKFB1), gene transcription and adipocyte differentiation (JUNB), as well as those involved in ECM remodeling and cell adhesion (Supplementary File 3).