TRIM33 and infection: The negative effect of TRIM33 was also confirmed in single-round HIV-1NL4-3.Luc.R-E- infection of the shRNA-transduced cells, in which luciferase activity was selectively increased in clones #4 and #5 in which the proteins had been knocked down (experimental scheme and results of luciferase acitivity in Supplementary Fig. 7h, i respectively).