After 7 days double antibiotic selection, we verified proper expression of the TRIM proteins in the different clones (representative western blot and RT-PCR quantification in Fig. 7e, f and respectively) and then infected these cells with wt HIV-1BRU; infection was monitored over a course of 12 days by measuring the amounts p24 viral protein production. This evidence concerns the gene TRAT1 and infection.