To obtain a more precise analysis of the dynamics of BRN2 recruitment to sites of DNA damage we performed live cell imaging of GFP-BRN2 expressed in U-2 OS osteosarcoma cells that are frequently used as a model system for examination of DNA repair (Kochan et al. 2017) and which do not express endogenous BRN2. This evidence concerns the gene POU3F2 and osteosarcoma.