To model the relevant esophageal interfaces, we used three epithelial cell lines: EPC2, an immortalised squamous epithelial cell line from the normal esophagus (Harada et al., 2003); CP-A, an immortalised BE cell line with gastric columnar epithelial properties (Merlo et al., 2011); and OE33, derived from EAC (Boonstra et al., 2010). The gene discussed is CPA1; the disease is Barrett esophagus.