This antibody has been verified for its specificity and applied to detect Tmem100 expression in mouse myenteric neurons and human hepatocellular carcinoma tissues in previous publications.16,45 Here, Tmem100 immunoreactivity (IR) stained the full profile of small- and middle-sized neurons, but in contrast displayed “ring-like” profiles in the case of large-sized neurons, in a pattern that could represent Tmem100 immunopositivity in neuronal membrane, SGCs, or both (Fig. 1A, B, B1). The gene discussed is TMEM100; the disease is hepatocellular carcinoma.