In fact, the best cut-off value for the GPC1 EV test was the diagnosis of pancreatic cancer if individuals present with less than 604 EVs per microliter when in fact EV counts should rise with cancer due to the increased abundance of cells expressing the biomarkers, thus revealing a lack of association of GPC1 EVs with pancreatic cancer. The gene discussed is GPC1; the disease is pancreatic neoplasm.