Combined analysis with GP2 was intended to improve the effectiveness of our EV-based test, however an even lower number of metastatic cancer patients exhibited elevated GPC1-GP2 EV counts compared to mean counts in BPD patients than GPC1 EV counts (2 of 30 patients compared to 9 of 30 patients) (Figure 2A). This evidence concerns the gene GPC1 and metastatic malignant neoplasm.