HMGB1 and infection: Given the marked necrotic fields in the liver sections from Gal-3 KO mice (Figure 2A,B) and the ability of MCMV to induce necroptosis (Upton et al., 2017), we examined markers of necroptotic death, HMGB1 in liver tissue homogenates and membrane expression of calreticulin on hepatocytes, 36 h after infection.