ABO and Alzheimer disease: By using fluorescence Ca2+ imaging of fura-2 loaded cells, we have evaluated the change in the Ca2+ content in the ER, SOCE, the Ca2+ depletion from ER induced by different physiological agonists and the coupling ER-mitochondria, in young (4–8 DIV, short-term) and aged (15–21 DIV, long term) hippocampal neurons cultured in vitro and exposed to Aβo as a model of AD and in vitro aging.