Measurement of autophagic levels by immunoblotting revealed lower levels of both BECLIN 1 and LC3-II in iPSC-ECs and HAECs exposed to hyperglycemia, further supported by reduced presence of autophagosomes compared with iPSC-ECs in normoglycemic condition (Figure 2D), demonstrating an impairment in autophagy. Here, BECN1 is linked to Hyperglycemia.