Our in vitro and in vivo data revealed that inhibition of the METTL3-m6A-CDCP1 axis resulted in the decreased proliferation, migration, and invasion of bladder cancer cells and the chemical-transformed uroepithelial cells, suggesting that the METTL3-m6A-CDCP1 axis is essential for the growth and maintenance of bladder cancer cells and transformed cells. Here, CDCP1 is linked to urinary bladder carcinoma.