EPHA2 and neoplasm: Although PBMC with CD19/CD3 BiTE did not exhibit cytolytic activity in CD19-negative tumor cell lines (U251, RERF, and A549), PBMC with EphA2/CD3 BiTE was cytotoxic to these cells expressing EphA2, particularly EphA2 high-expressing U251 cells (Supplementary Fig. S4A,B).