The mean tumor weight was greatest in the untreated controls (1.24 ± 0.16 g); it was 1.05 ± 0.13 g in the anti-PD1 only group, 0.63 ± 0.05 g in the IRE only group, and 0.30 ± 0.02 g in the IRE + anti-PD1 group (mean ± SEM, n = 5 or 6, Fig. 3a). This evidence concerns the gene PDCD1 and neoplasm.