To determine whether the caspases were involved in gukulenin A-induced apoptosis in human ovarian cancer cells, the activation of caspase-3, -8, and -9 was evaluated after treatment with gukulenin A. Western blot analysis showed that gukulenin A treatment increased the levels of the cleaved forms of caspase-3, -8, and -9 in A2780 cells (Figure 5A). The gene discussed is CASP3; the disease is ovarian carcinoma.