Purification and cycle sequencing of the melanoma-derived 1253 bp PCR band and suitable alignment of the unveiled sequence with the reference one extracted from the Ensembl Genome Browser 91 proved the presence of a 912 bp long DNA sequence that corresponds to the intron 9/10 (912 bp) of Sestrin-1 gene (ENST00000436639.6) (Figure 3C). This evidence concerns the gene SESN1 and melanoma.