We previously found that stimulation of NOD2 aggravated stroke outcomes.5 To evaluate the effect of ARRB2 on NOD2‐induced lesion in cerebral I/R, MDP the extrinsic ligand of NOD2 was intraventricularly administered 30 minutes before MCAO to WT and ARRB2−/− mice. Here, ARRB2 is linked to stroke disorder.