In this study, we first demonstrated that MARCH1 was aberrantly and highly expressed in HCC samples and cells lines and that the knockdown expression of MARCH1 led to a dramatic decrease in the proliferation, migration, invasion and increase in apoptosis via the regulating of PI3K and its downstream AKT‐β‐catenin pathway. The gene discussed is AKT1; the disease is hepatocellular carcinoma.