The Western blotting showed a down‐regulated expression of MARCH1, PI3K, P‐AKT, β‐catenin, Bcl‐2 and Mcl‐1 and an up‐regulated expression of pro‐apoptosis‐related Cleaved caspase‐3 and Cleaved caspase‐7 in the HCC tumours injected with MARCH1 siRNA compared with the negative controls (Figure 6E). This evidence concerns the gene MARCHF1 and hepatocellular carcinoma.