Methylation rates were observed between 22 and 68% for PAX5 gene and between 37 and 51% of patients for TMPRSS2 gene in tissue of patients with malignant melanoma according to ß-actin gene (Figure 3) (Table 6). PAX5 gene was found methylated in all patients with malignant melanoma [6/6, (100%)] for primary tumor tissues and each type of culture (Table 5). TMPRSS2 was observed as methylated in 2D and 3D culture in 4 samples [4/6, (67%)] and in tumor tissue samples of 5 patients [5/6, (83%)] (Table 5). Here, TMPRSS2 is linked to melanoma.