Not only are LGR5 and ASCL2 robust stem cell markers, but numerous studies have shown them to be upregulated in CRCs, with many reporting that they are markers for CRC stem cells (Merlos-Suárez et al., 2011; Kemper et al., 2012; Kobayashi et al., 2012; Hirsch et al., 2014; Jubb et al., 2006; Ziskin et al., 2013; Shimokawa et al., 2017). This evidence concerns the gene ASCL2 and colorectal carcinoma.