We established such a subline from a primarily regressed and further relapsed PD-1-sensitive cell line-derived MC38-CEA tumor (Doc.S3 sMDI, Figure S3 sMDI), which not only changed the antigenic profile (loss of CEA expression), but also became completely PD-1-resistant (Doc.S3 sMDI, Figure S3 sMDI). This evidence concerns the gene PDCD1 and neoplasm.