To this end, and also to assess whether the fused and the non-fused BoNT-A LC proteins were able to cleave SNAP25, primary cultures of rat embryonic DRG neurons were infected by vectors GFP-BoNT-A LC, BoNT-A LC, or Luc (the two latter vectors also expressing GFP as a free protein) at increasing multiplicities of infection (MOI), from 0.1 to 10.0 transducing units (TU)/neuron. Here, SNAP25 is linked to infection.