Thus, the aim of the present study was to assess (i) the association between maternal LINE-1 methylation and the occurrence of CHD in children with DS and (ii) the association of endogenous maternal factors (MTHFR C677T polymorphism and maternal age), and exogenous maternal factors (cigarette smoking, alcohol intake, medication use, body mass index, and dietary habits, such as folate intake) with LINE-1 methylation in mothers of children with DS and CHD. Here, MTHFR is linked to coronary artery disorder.