To verify whether the reduction of CCDC6 levels, induced by P5091, altered the double strand breaks (DSBs) DNA repair by homologous recombination (HR), the bladder cancer cells pretreated with P5091, or untreated, were transfected with the DR-GFP reporter plasmid alone, as a control, or together with the I-SceI plasmid, able to induce DSBs. This evidence concerns the gene CCDC6 and urinary bladder carcinoma.