Consistently, the expression of miR-335 and MEST are highly correlated in TCGA HCC (R = 0.88; FDR = 9.87e-05), breast (R = 0.78; FDR = 0) and gastric cancer (R = 0.79; FDR = 0), further validating of the co-regulation relationship between miR-335 and MEST is needed. The gene discussed is MEST; the disease is hepatocellular carcinoma.