ESR1 and breast cancer: To explore this hypothesis a high resolution chromatin interaction analysis of this region in breast cancer cells would be required, such as 5C61 or NG Capture-C,62 coupled with ERα ChIP-Seq and ChIA-PET.29 In addition, we see an increase in MIR196A2 expression in response to low-dose E2, suggesting a direct influence by ERα that is further increased at higher dosages of 10 nM in our qRT-PCR assays.