To investigate whether RAS mutational status influences the effect of autophagy in regulating EMT, we used RNA interference (RNAi) to deplete ATG5, an important component of the autophagic machinery, in 7 human cancer cell lines derived from the pancreas (PaCa3, Suit-2, PANC1 and MDA Panc3) and colon (HCT116, HKe3 and HKh2). This evidence concerns the gene ATG5 and cancer.