Downstream targets of miRNA-486-5p include Phosphoinositide-3-Kinase Regulatory Subunit 1 (PIK3R1) and Never in Mitosis gene a-(NIMA-)Related Kinase 2 (NEK2), which have been shown to play important roles in HCC proliferation, migration, and invasion [23,24]. The gene discussed is PIK3R1; the disease is hepatocellular carcinoma.