This finding is in partial agreement with previous studies that showed that GFAP mRNA was dramatically increased in the hippocampus in the first few days after 10 min of tgCI in gerbils [22] and that morphologically activated astrocytes began to increased 7 days after 10 min of global cerebral ischemia, showing that weakly reactive astrocytes were observed in the CA3 region in rats [10,23]. Here, GFAP is linked to brain ischemia.