Our study demonstrated that the activation levels of NF-κB (p65), pro-caspase-1, pro-IL-1β, NLRP3, caspase-1 and IL-1β were increased after CLP/LPS+ATP, illustrating that the NLRP3/caspase-1/IL-1β signaling pathway participates in sepsis. The gene discussed is CASP1; the disease is Sepsis.