To assess the relationship between NET formation and tumor cell motility, we performed a coculture transwell migration assay (the details are provided in the “Materials and Methods” section, Fig. 6a) with two cell lines: MDA-MB-231 cells, which show a high production of G-CSF, and MCF-7, which rarely secretes G-CSF (Fig. 6b, c). This evidence concerns the gene CSF3 and neoplasm.